Sterilization method for affinity chromatography matrices

• Main Authors: MONEY ELIN MARIANNE, BJORKMAN TOMAS, LJUNGLOF ANDERS, TORSTENSSON KARIN, GRONBERG ANDREAS, WETTERHALL MARK CORNELIS ELISABETH, RODRIGO GUSTAV JOSE
• Format: Patent
• Language: Chinese; English
• Published: 25.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES; CHEMISTRY; DISINFECTION, STERILISATION, OR DEODORISATION OF AIR; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES; MEDICAL OR VETERINARY SCIENCE; METALLURGY; METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PEST REPELLANTS OR ATTRACTANTS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; SEPARATION; TRANSPORTING; TRAPPING

The invention discloses a method for cleaning or disinfecting an affinity chromatography matrix. Comprising the following steps: a) providing an affinity chromatography matrix having an oxidation resistant proteinaceous ligand coupled to a support, b) contacting the matrix with a disinfection solution containing at least one oxidizing agent defined by Formula I, R-O-O-H (I) wherein R is hydrogen or acyl R '-C (O)-and R' is hydrogen or methyl, ethyl or propyl. 本发明公开了一种用于亲和色谱基质清洗或消毒的方法，包括以下步骤：a）提供一种具有偶联到载体的耐氧化蛋白质类配体的亲和色谱基质，b）使所述基质与含有至少一种由分子式I定义的氧化剂的消毒溶液接触，R-O-O-H(I)，其中，R为氢或酰基R'-C(O)-，R'为氢或甲基、乙基或丙基。