Kit and method for detecting BRCA1 gene mutation

• Main Authors: LIU QUAN, WEN XIAOSHA, LUO DIXIAN
• Format: Patent
• Language: Chinese; English
• Published: 21.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a kit and a method for detecting BRCA1 gene mutation. The kit comprises a sense primer, an antisense primer and a high-temperature-resistant restriction enzyme, the sense primer and the antisense primer are primers respectively combined with the upstream and the downstream of the c.981982 region of the BRCA1 gene, and the high-temperature-resistant restriction enzyme is used for cutting a wild type fragment of the c.981982 region of the BRCA1 gene. According to the method, PCR amplification and restriction enzyme digestion are ingeniously combined, a method for enriching BRCA1 gene mutation region fragments is developed, rapid enrichment can be achieved, the product can be directly subjected to follow-up detection, the detection sensitivity of low-abundance mutation can be improved, operation is easy and convenient, cost is low, additional equipment does not need to be added, and wide application and popularization are achieved. 本发明公开了一种检测BRCA1基因突变的试剂盒及方法。所述试剂盒包括正义引物、反义引物和耐高温限制性内切酶，所述正