Primer combination for detecting 32 viruses and amplicon targeting sequencing method

• Main Authors: GU LIJIANG, XIA MEILING, YI KANG
• Format: Patent
• Language: Chinese; English
• Published: 21.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer combination for detecting 32 kinds of viruses, the primer combination comprises an upstream primer F1: 5 '-GAACGACATGCTACGATCCGACTT specific F-3' and a downstream primer R1: 5 '-GACCGCTTGCCTCGACTT specific R-3', specific F comprises a singular sequence in a sequence 1 to a sequence 126 in a sequence table, and specific R comprises an even sequence in the sequence 1 to the sequence 126 in the sequence table. According to the method, whether one or more of 32 viruses exist in a sample or not can be rapidly identified, the sample detection limit can be as low as 5 ng, the detection accuracy is high and is 95% or above, and no carrying pollution exists. Two types of nucleic acids of DNA and RNA can be detected at the same time, reverse transcription of RNA and amplification of DNA are completed in the same reaction, and the operation process is greatly simplified. 本发明公开了一种用于32种病毒检测的引物组合，包括上游引物F1：5'-GAACGACATGGCTACGATCCGACTT special F-3'，下游引物R1：5'-GACCGCTTGGCCTCCGACTT special R-3'，