TaqMan fluorescent quantitative PCR (polymerase chain reaction) method for rapidly detecting prawn blood cell iridovirus

• Main Authors: LIU JIAYU, CHEN TING, CHE LUPING, QIN LITING, LI XIAOFEI
• Format: Patent
• Language: Chinese; English
• Published: 18.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) method for rapidly detecting a prawn blood cell iridovirus, and belongs to the technical field of virus PCR detection. The PCR method provided by the invention comprises the following steps: firstly designing and synthesizing a primer and a probe, then obtaining a DNA template of a sample to be detected, and finally preparing a TaqMan PCR reaction system for PCR reaction. The PCR method has the characteristics of high sensitivity and strong specificity, and the lowest detection limit can reach 1 copy/mu L. 本发明提供了一种快速检测对虾血细胞虹彩病毒的TaqMan荧光定量PCR方法，属于病毒PCR检测技术领域。本发明所提供的PCR方法首先设计合成引物和探针，然后获取待测样品DNA模板，最后配制TaqMan PCR反应体系进行PCR反应。该PCR方法具有高灵敏度和特异性强的特点，其最低检测限可达到1 copy/μL。