Streptococcus bovis primer group based on 16s rRNA and LAMP detection method thereof

• Main Authors: CHEN YUZUO, SUOLANGSIZHU, WANG BAONING, LIU RENJIE
• Format: Patent
• Language: Chinese; English
• Published: 18.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a streptococcus bovis primer group based on 16s rRNA (ribosomal Ribonucleic Acid) and an LAMP (Loop-Mediated Isothermal Amplification) detection method thereof, and relates to the technical field of rapid detection of molecular diagnosis. The primer group comprises a primer F3-3, a primer B3-3, a primer FIP-3, a primer BIP-3, a primer FL-3 and a primer BL-3; the nucleotide sequences of the primer F3-3, the primer B3-3, the primer FIP-3, the primer BIP-3, the primer FL-3 and the primer BL-3 are respectively as shown in SEQ ID No. 13 to SEQ ID No. 18; the LAMP detection method comprises the detection reagent, reaction conditions and the primer group. The specific streptococcus bovis LAMP detection method established by using the streptococcus bovis conservative sequence 16s rRNA design primer can quickly complete specific amplification within 30-60 minutes without a special PCR amplification instrument on a detection site, the result is visible by naked eyes, and the specific streptococcu