Application of circular RNA circ0000615 as marker in preparation of colorectal cancer detection kit

• Main Authors: ZHANG XINYU, HU YUHAN, ZHONG JIATENG, ZHAO YAO, SU WEI, GAO QINGZU, DING SHUANG
• Format: Patent
• Language: Chinese; English
• Published: 18.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of molecular markers, and provides application of circular RNAcirc0000615 as a marker in preparation of a colorectal cancer detection kit. The nucleotide sequence of the circular RNA circ0000615 disclosed by the invention is as shown in SEQ ID NO. 1 (sequence identifier number 1). The research result shows that the expression level of the circular RNAcirc0000615 is in negative correlation with the colorectal cancer, and the circular RNAcirc0000615 is RNA with remarkably reduced expression in CRC tissue and can be used as a diagnosis marker of cancer. Meanwhile, after overexpression of the circular RNAcirc0000615, the multiplication capacity of colorectal cancer can be remarkably inhibited, the occurrence and development of tumors can be inhibited, and the circular RNAcirc0000615 can be used as a potential way for cancer treatment. 本发明属于分子标志物技术领域，本发明提供了环状RNAcirc_0000615作为标志物在制备结直肠癌检测试剂盒中的应用。本发明所述环状RNA circ_0000615的核苷酸序列如SEQ ID NO.1所示。本发明的研究结果表明，环状RNAcirc_0000615的表达水