Preparation method of corn autotetraploid

• Main Authors: ZHONG CHANGSONG, LU SHENGQIAO, TAN HONGYU, WEI ZHENGYI, GONG XUE, ZHOU JINGUO, SU QI, ZHANG SHUKUAN, LIU YALI, CHEN KUN
• Format: Patent
• Language: Chinese; English
• Published: 18.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention belongs to the technical field of plant breeding, and particularly relates to a manufacturing method of a corn autotetraploid. The preparation method of the corn autotetraploid comprises the following steps: (1) disinfection; (2) embryo stripping; (3) rooting; (4) seedling formation; (5) seedling hardening; and (6) identifying. According to the method, corn immature embryos are used as induction materials, cells in the immature embryo stage are in an early differentiation state, autotetraploid is more easily formed than in the germination stage and the seedling stage, the breeding period can be effectively shortened, and annual creation of tetraploid is achieved. 本发明属于植物育种技术领域，具体涉及一种玉米同源四倍体的制作方法。一种玉米同源四倍体的制作方法，包括以下步骤：(1)消毒；(2)剥胚；(3)生根；(4)成苗；(5)炼苗；(6)鉴定。本发明以玉米幼胚为诱导材料，幼胚阶段细胞处于早期分化状态，比萌发期和幼苗期更易形成同源四倍体，可有效缩短育种年限，实现四倍体周年创制。