Method for detecting infectious muscular necrosis viruses of prawns based on ERA technology

• Main Authors: HU JINGJIE, ZHOU QINGQIAN, WANG MENGQIANG, LIU KEXIN, SHAO SHUORU, BAO ZHENMIN, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 14.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a prawn infectious muscle necrosis virus detection method based on an ERA technology. According to the present invention, the MCP sequence in the prawn infectious muscular necrosis virus gene is selected as the detection target gene, the special primer and the probe for the ERA detection are designed and synthesized according to the target gene, and the ERA detection method for the prawn infectious muscular necrosis virus is established; the method is fixed in reaction temperature, simple in operation procedure, high in amplification speed, high in detection efficiency, good in sensitivity and specificity and suitable for rapid detection of IMNV. 本发明提供一种基于ERA技术的对虾传染性肌肉坏死病毒的检测方法。本发明选取对虾传染性肌肉坏死病毒基因中MCP序列作为检测靶基因，针对靶基因设计合成ERA检测专用引物和探针，建立用于对虾传染性肌肉坏死病毒的ERA检测方法。本发明方法反应温度固定、操作程序简单、扩增速度快、检测效率高，且灵敏性和特异性良好，适用于IMNV的快速检测。