Pear transcription factor PyERF4.1 gene for inhibiting anthocyanin biosynthesis as well as recombinant expression vector and application of pear transcription factor PyERF4.1 gene

• Main Authors: GOU SHASHA, XIAO YUWEI, YAO GAIFANG, HU KANGDI, ZHANG HUA, SUN HONGYE
• Format: Patent
• Language: Chinese; English
• Published: 14.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a pear transcription factor PyERF4.1 gene for inhibiting anthocyanin biosynthesis as well as a recombinant expression vector and application of the pear transcription factor PyERF4.1 gene. The nucleotide sequence of the PyERF4.2 gene is shown as SEQ ID No.1. According to the application, a transcription factor PyERF4.1 is over-expressed and knocked out from pear calluses and tomato fruits, the function of inhibiting anthocyanin biosynthesis of the transcription factor PyERF4.1 is verified, pear fruits and strawberry fruits are co-transformed by PyERF4.1, PyERF3, PyMYB114 and PybHLH3 genes, so that accumulation of anthocyanin in the pear fruits and the strawberry fruits is reduced, and biological function verification shows that the PyERF4.1, PyERF3, PyMYB114 and PybHLH3 genes can be used for inhibiting the anthocyanin biosynthesis of the pear fruits and the strawberry fruits. The invention shows that the cloned PyERF4.1, the PyERF3 gene, the PyMYB114 gene and the PybHLH3 gene interact