Preparation and application of nano antibody for typing detection of helicobacter pylori

• Main Authors: PENG SINAN, MA JINGWEI, TANG FENG, XU JIN, LI RUNLE, GUO LE, LIU WENJING, XIN MINGYUAN
• Format: Patent
• Language: Chinese; English
• Published: 14.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The invention belongs to the technical field of biological medicines, and discloses preparation and application of a nano antibody for typing detection of helicobacter pylori. Based on a phage nano antibody display library, the nano antibody for helicobacter pylori cytotoxin related protein CagA and urease B subunits is obtained through multiple rounds of screening; elisa verifies that the Helicobacter pylori cytotoxin-related protein CagA and urease B subunit can be specifically recognized, and the binding activity is relatively high. The invention also provides an expression vector containing the coding sequence of the variable region of the nano antibody and a host cell containing the expression vector, a recombinant genetic engineering strain is successfully constructed, and the target protein is obtained after expression and purification. The nano antibodies of the CagA subunit and the urease B subunit respectively have obvious specific binding capacity to the CagA subunit and the urease B subunit, can b