HapDR related to identification of drought tolerance in corn seedling stage and application of HapDR

• Main Authors: ZHANG FENGQI, TANG BAOJUN, ZHANG JUN, XIA LAIKUN, MA ZHIYAN, QI JIANSHUANG, MU XINYUAN, DING YONG, LU YUANFANG
• Format: Patent
• Language: Chinese; English
• Published: 11.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of genetic breeding and molecular biology, and discloses haplotype ZmC10.HapDR related to identification of drought tolerance in a corn seedling stage and application of the haplotype ZmC10.HapDR. Zheng A88 and Zheng T22 are hybridized to construct a segregation population, and gene localization is carried out by combining a genetic offspring mixed pool re-sequencing (BSA-seq) and a transcriptome sequencing (RNA-seq) method, so that the haplotype ZmC10.HapDR related to control of drought tolerance in the corn seedling stage is obtained. According to the invention, the selection efficiency is improved, the haplotype ZmC10.HapDR site contribution is great, the detection is convenient and rapid, the drought-resistant material in the seedling stage can be rapidly screened for the drought-resistant genetic improvement of corn varieties, the breeding selection target is clear, and the cost is saved. 本发明涉及遗传育种和分子生物学技术领域，公开了鉴定玉米苗期耐旱相关的单体型ZmC10.HapDR及其应用。利用郑A88与郑T22进行杂交构建分离