Acute hepatopancreatic necrosis disease pathogen detection method based on ERA technology and application

• Main Authors: HU JINGJIE, ZHOU QINGQIAN, WANG MENGQIANG, LIU KEXIN, SHAO SHUORU, BAO ZHENMIN, WANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 11.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an acute hepatopancreatic necrosis disease pathogen detection method based on an ERA technology and application, and the method comprises the following steps: (1) taking a piAB sequence with the length of 1665bp in acute hepatopancreatic necrosis disease pathogen plasmids as a detection target gene, and designing and screening an RT-ERA primer group and an RT-ERA fluorescent probe; (2) taking the genome DNA of a sample to be detected as a template, carrying out RT-ERA amplification at a constant temperature of 33-42 DEG C by using the designed RT-ERA primer group and the probe, detecting the fluorescence signal intensity of an RT-ERA amplification product, and judging an amplification result according to a fluorescence signal value of an amplification curve within 15 minutes; a real-time quantitative enzymatic recombinant amplification technology RT-ERA is utilized to establish an AHPND detection system, efficient amplification can be performed at 33-42 DEG C, a detection result can be