Method for improving secretion rate of foreign protein expressed by bombyx mori cells

• Main Authors: HUANG JINSHAN, HOU YUANLIN, HUO JIAYAO, YAO CONGYUE, LU MENGZE, ZHAO YATING, HAO BIFANG
• Format: Patent
• Language: Chinese; English
• Published: 11.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a method for improving the secretion rate of foreign protein expressed by bombyx mori cells, which comprises the following steps of: extracting cDNA (complementary deoxyribonucleic acid) obtained by reverse transcription of all RNA (ribonucleic acid) of bombyx mori BmN cells, synthesizing an spz gene by taking the cDNA obtained by reverse transcription as a template, and transferring the spz gene shown as SEQ ID NO.1 into the bombyx mori BmN cells, so that the secretion rate of the foreign protein expressed by the bombyx mori cells can be improved. The method can effectively improve the secretion efficiency of the foreign protein expressed in the bombyx mori cells, provides a new research direction and thought for improving the secretion rate of the foreign protein expressed in the bombyx mori cells, and provides reference for mechanism research of signal peptide participating in protein secretion. 一种提高家蚕细胞表达外源蛋白分泌率的方法，提取家蚕BmN细胞的全部RNA反转录得到的cDNA，以反转录得到的cDNA为模板合成spz基因，再将SEQ ID NO.1所示的sp