Lysate for extracting DNA based on paramagnetic particle method, kit and extraction method

• Main Authors: GUO RENKANG, JIANG RUFEI, SONG JIANJUN, YANG XIAOLAN, PAN CHAO
• Format: Patent
• Language: Chinese; English
• Published: 11.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a lysis solution, a kit and an extraction method for extracting DNA (deoxyribonucleic acid) based on a paramagnetic particle method, and the lysis solution comprises sodium chloride, Tween-20, ethylenediamine tetraacetic acid and guanidine isothiocyanate; the concentration of sodium chloride in the lysate is 2-3 mol/L, the volume fraction of Tween-20 is 10%-20%, the lysate containing high-concentration sodium chloride and Tween-20 can replace protease K, nucleic acid and histone can be separated without additionally adding the protease K, and DNA is dissociated. When the DNA is extracted by adopting the paramagnetic particle method provided by the invention, protease K addition and heating are not needed when the blood genome DNA is extracted, the high-purity DNA can be extracted under the condition of normal temperature, and the detection result is more accurate. The DNA extraction kit prepared by using the lysate provided by the invention can extract complete DNA of a blood genome at