SERS (Surface Enhanced Raman Scattering) bionic taste sensing chip for cell secretions as well as preparation and detection methods

• Main Authors: CUI YIPING, ZONG SHENFEI, WU LEI, WANG ZHUYUAN, CHEN LU
• Format: Patent
• Language: Chinese; English
• Published: 07.04.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a cell secretion-oriented SERS bionic taste sensing chip and a preparation and detection method thereof, the biological detection chip is composed of an SERS sensing array and a fixing device thereof, the chip can be directly suspended on a cell culture dish, a clamping groove beside the chip is used for fixing a sensor substrate, and the SERS sensing array is used for fixing the SERS sensing array. The SERS sensing array assembly on the substrate is composed of a metal nanoparticle compact single layer and an acceptor molecule array, and cell secretions can be quantitatively detected by collecting and analyzing SERS spectrums of array units. The device can be matched with a cell culture dish, and real-time, high-sensitivity and automatic quantitative analysis of various extracellular secretions to be detected is realized under the condition that cell culture is not influenced. 本发明公开了一种面向细胞分泌物的SERS仿生味觉传感芯片及其制备、检测方法，所述生物检测芯片由SERS传感阵列及其固定装置组成，该芯片可直接悬挂在细胞培养皿上，旁边的卡槽用于固定传感器基片，基片上的SERS传感阵列组装