Mixed plasmid, standard plasmid and application thereof

• Main Author: CAO JINLIANG
• Format: Patent
• Language: Chinese; English
• Published: 07.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a mixed plasmid, a standard plasmid and application thereof, and belongs to the technical field of biology. The sequence of the mixed plasmid is shown as SEQ ID NO: 1 in a sequence table. The standard plasmid comprises a plasmid vector and a mixed plasmid inserted into the plasmid vector. According to the application, the standard plasmid is used as a positive quality control for detecting the IDH1 gene mutation type. The mixed plasmid provided by the embodiment of the invention contains six IDH1 gene mutation types, and the standard plasmid obtained by utilizing the mixed plasmid can be used as a positive standard quality control product and is used for determining the six IDH1 gene mutation types in a human tumor sample in two times of detection, so that the detection efficiency is effectively improved, and the detection time is shortened. And the risk of pollution caused by manual operation errors and multiple experiments is reduced. 本发明提供了一种混合质粒、标准质粒及其应用，属于生物技术领域。所述混合质粒的序列如序列表中SEQ I