Compositions and methods for modifying genomes

• Main Authors: GRAY BENJAMIN NEIL, BEGEMANN MATTHEW
• Format: Patent
• Language: Chinese; English
• Published: 07.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Compositions and methods for modifying genomic DNA sequences are provided. The method generates a double strand break (DSB) at a predetermined target site in a genomic DNA sequence, at which target site results in a mutation, insertion and/or deletion of the DNA sequence. Compositions include a DNA construct comprising a nucleotide acid sequence encoding a Cpf1 or Csm1 protein operatively linked to a promoter operative in a cell of interest. The DNA constructs can be used to direct genomic DNA modification at a predetermined genomic locus. Described herein are methods of using these DNA constructs to modify genomic DNA sequences. In addition, compositions and methods for modulating gene expression are provided. Compositions include a DNA construct comprising a promoter operative in a cell of interest operatively linked to a nucleotide sequence encoding a mutant Cpf1 or Csm1 protein that eliminates DSB-producing ability, optionally linked to a domain that modulates transcriptional activity. The methods can be