DNA (deoxyribonucleic acid) framework for promoting circRNA (ribonucleic acid) cyclization and overexpression as well as construction method and application of DNA framework

• Main Authors: PENG DAZHAO, WEI CHENG, HAN LEI
• Format: Patent
• Language: Chinese; English
• Published: 07.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a DNA framework for promoting circRNA cyclization and overexpression and a construction method and application thereof, the DNA framework comprises an upstream cyclization driving sequence, a target circRNA gene sequence and a downstream cyclization driving sequence, the sequence of the upstream cyclization driving sequence is shown as SEQ ID NO: 1, and the sequence of the downstream cyclization driving sequence is shown as SEQ ID NO: 2. The intron flanking complementary sequence provided by the invention is necessary for circUACA cyclization, and the complementary sequence can effectively promote the overexpression of circUACA, and the overexpression efficiency can be as high as 500 times. 本发明提供了一种用于促进circRNA成环和过表达的DNA框架及其构建方法和用途，所述DNA框架包含上游环化驱动序列-目的circRNA基因序列-下游环化驱动序列，所述上游环化驱动序列的序列如SEQ ID NO：1所示，所述下游环化驱动序列如SEQ ID NO：2所示。本发明所述的内含子侧翼互补序列是circUACA环化所必需的，并且该互补序列可以有效促进circUACA的过表达，过表达效率可高达到500倍。