Application of protein ZmRLK9 in regulation and control of corn kernel embryo development and oil content

• Main Authors: JIN WEIWEI, WANG TIANYI, HUANG WEI, DONG CHAOBIN, LI YUNFEI
• Format: Patent
• Language: Chinese; English
• Published: 07.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses an application of protein ZmRLK9 in regulation and control of corn kernel embryo development and oil content. The amino acid sequence of the protein ZmRLK9 is as shown in SEQ ID NO: 1. Experiments prove that the ZmRLK9 gene is over-expressed in corn to obtain transgenic corn; compared with the starting corn, the embryo area of the transgenic corn and the oil content of mature grains are obviously increased. The ZmRLK9 gene is inhibited in corn, and the ZmRLK9 gene knockout rice is obtained; compared with starting corn, the rice with the ZmRLK9 gene knocked out has the advantage that the embryo area and the oil content of mature grains are remarkably reduced. Therefore, the protein ZmRLK9 can regulate and control the corn kernel embryo development and the oil content. The method has an important application value. 本发明公开了蛋白质ZmRLK9在调控玉米籽粒胚发育和油分含量中的应用。蛋白质ZmRLK9的氨基酸序列如SEQ ID NO:1所示。实验证明，在玉米中过表达ZmRLK9基因，得到转基因玉米；与出发玉米相比，转基因玉米的胚面积和成熟籽粒的油分含量均显著增加。在玉米中抑制ZmRLK9基因，得到敲除ZmRLK9基因水稻；与出发玉米相比，敲除ZmRLK9基因