Method for extracting sulforaphane through step-by-step enzymolysis by combining cellulase with myrosinase

The invention discloses a method for extracting sulforaphane through step-by-step enzymolysis by combining cellulase with myrosinase. The method is characterized by comprising the following steps: S1, pretreating a sample; s2, cellulase enzymolysis; s3, enzyme deactivation; s4, myrosinase enzymolysi...

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Bibliographic Details
Main Author MAO WEIBIN
Format Patent
LanguageChinese
English
Published 04.04.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for extracting sulforaphane through step-by-step enzymolysis by combining cellulase with myrosinase. The method is characterized by comprising the following steps: S1, pretreating a sample; s2, cellulase enzymolysis; s3, enzyme deactivation; s4, myrosinase enzymolysis: myrosinase and a catalyst are added into the first enzymatic hydrolysate, and enzymolysis is performed at the temperature of 40-42 DEG C for 8-15 h; s5, extracting sulforaphane: adding an extraction solvent into the enzymatic hydrolysate after enzymolysis in the step S4, applying ultrasonic treatment, and extracting for 30-60 minutes under the condition that the temperature is 40-60 DEG C; s6, filtering; and S7, concentrating: concentrating the filtrate obtained in the step S6 until the extraction solvent is completely volatilized to obtain an oily sulforaphane product. According to the method disclosed by the invention, the cellulase is adopted to pretreat the raw materials, then the myrosinase is utilized to p
Bibliography:Application Number: CN202211478901