Sclerotinia sclerotiorum SsCAK1 protein as well as coding gene and application thereof

• Main Authors: XIA SHITOU, TAN LEI
• Format: Patent
• Language: Chinese; English
• Published: 04.04.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a sclerotinia sclerotiorum SsCAK1 protein as well as a coding gene and application thereof. The amino acid sequence of the sclerotinia sclerotiorum SsCAK1 protein is shown as SEQ ID NO. 1. A target silent sclerotinia sclerotiorum SsCAK1 gene RNAi sequence is designed and synthesized, the RNAi sequence for inhibiting or silencing sclerotinia sclerotiorum SsCAK1 gene expression is used for constructing and obtaining a silent expression vector, after the silent expression vector is transformed into a plant, the plant can have sclerotinia sclerotiorum resistance, and the silent expression vector has a huge application prospect in plant sclerotinia sclerotiorum resistance breeding. 本发明公开了核盘菌SsCAK1蛋白及其编码基因与应用，所述核盘菌SsCAK1蛋白氨基酸序列如SEQ ID NO.1所示。通过设计合成靶向沉默核盘菌SsCAK1基因RNAi序列，将抑制或沉默核盘菌SsCAK1基因表达的RNAi序列用于构建并得到沉默表达载体，将其转化植物后，可以使得植物具有菌核病抗性，其在植物菌核病抗性育种具有巨大的应用前景。