HIV-1 p24 ELISA (enzyme-linked immunosorbent assay) detection kit as well as use method and application thereof

• Main Authors: ZHANG TIANYANG, ZHENG MENGTAO, FAN PENGCHENG, LI ZHA, CAI YUQING, LIU LIMEI
• Format: Patent
• Language: Chinese; English
• Published: 31.03.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of biology, in particular to the field of IPCG01N33, and more particularly relates to an HIV (human immunodeficiency virus)-1p24 ELISA (enzyme-linked immunosorbent assay) detection kit as well as a use method and application thereof. The HIV-1p24 protein in a sample is detected by adopting a double-antibody sandwich method, a double-antibody coated enzyme-linked plate is used, and a biotin-calibrated p24 antibody is used as a detection antibody to complete the development of the kit. And the plate coating does not need to be carried out one day before, the workload of an operator is reduced, and the whole operation time is saved. The quantitative range of the kit is 6.25-200 pg/mL, and LOD is less than or equal to 3.125 pg/mL. Through verification, the detection method of the kit is high in sensitivity, has good precision and accuracy, and can be used for detecting the content of p24 protein in a virus sample. 本发明涉及生物技术领域，尤其涉及IPCG01N33领域，更具体地，涉及一种HIV-1p24ELISA检测试剂盒