Method for analyzing concentration of proton pump inhibitor rabeprazole in plasma sample by using stable isotope internal standard

• Main Authors: WANG BEIBEI, GAO YAN, LIN HAIXIA, ZHONG KAN, LI HANG, CHOI YEO-OK
• Format: Patent
• Language: Chinese; English
• Published: 31.03.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for analyzing the concentration of a proton pump inhibitor rabeprazole in a plasma sample by using a stable isotope internal standard. The method comprises the following steps: adding a stable isotope internal standard solution and methanol into a plasma sample, carrying out vortex, centrifuging, collecting a supernatant, adding methanol and acetonitrile into the supernatant, and carrying out vortex mixing to obtain a pretreated sample to be detected; the method comprises the following steps: carrying out liquid chromatography-tandem mass spectrometry detection on a to-be-detected sample, carrying out mass spectrometry detection, drawing a standard curve based on a detection peak area ratio to obtain a regression equation, and finally calculating to obtain the rabeprazole concentration in the to-be-detected sample. The method has the characteristic of simple and convenient pretreatment operation, the extracted sample is easy to store, the analysis speed is high, and the method