Epigenetic marker for screening and/or detecting sperm motility of breeding bull and application of epigenetic marker

• Main Authors: YANG CHUNHONG, WEI XIAOCHAO, JIANG QIANG, HUANG JINMING, WANG XIUGE, WANG JINPENG, ZHANG YARAN, XIAO YAO, GAO YAPING, JU ZHIHUA
• Format: Patent
• Language: Chinese; English
• Published: 31.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of epigenetic markers related to sperm motility of bulls, and particularly relates to an epigenetic marker for screening and/or detecting sperm motility of breeding bulls and application of the epigenetic marker. Aiming at DNA methylation differences and functions of sperm genomes of full-sib Holstein bulls with high and low sperm motility, simplified methylation sequencing and transcriptome sequencing are respectively carried out on sperm genome DNA and total RNA, and a molecular mechanism of DNA methylation playing a role in bull sperm motility is discussed. It is determined that the high methylation level of the 29th exon region of the PBRM1 gene is related to the high sperm activity of the bull. And the methylation level of the 29th exon of the PBR M1 gene is further determined, and expression and functions of the gene are regulated and controlled by changing a variable splicing mode, so that a foundation is laid for screening high-sperm motility bulls, genetic