Primer probe group, kit and detection method for detecting PIK3CA gene mutation

• Main Authors: WU PEIYANG, HUANG YAN, LIAN SHIWEN, GE GUANGJUN
• Format: Patent
• Language: Chinese; English
• Published: 28.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer probe group, a kit and a detection method for detecting PIK3CA gene mutation. The primer probe group comprises a first primer, a second primer, a first probe, a second probe and a third probe; wherein the sequence of the first primer comprises a sequence as shown in SEQ ID NO.1, the sequence of the second primer comprises a sequence as shown in SEQ ID NO.2, the sequence of the first probe comprises a sequence as shown in SEQ ID NO.3, the sequence of the second probe comprises a sequence as shown in SEQ ID NO.4, and the sequence of the third probe comprises a sequence as shown in SEQ ID NO.5. By screening multiple groups of primer probes and kicking out interference of pseudogenes on results, the problem that a traditional fluorescent quantitative PCR solution has false positive is solved, and more accurate postoperative tracking and medication guidance can be provided for patients. 本发明公开了用于检测PIK3CA基因突变的引物探针组及试剂盒和检测方法。所述引物探针组包括第一引物、第二引物、第一探针、第二探针和第三探针；其中，所述第一引物的序列包括如SEQ ID NO.1