Primer group and method for detecting mutation of 108 polymorphic sites of deafness related gene

• Main Authors: WANG HUAWEN, GAN LIJIA, GUO CILIN, SUN MINGMING, HU CHANGMING, TANG ZHUJING
• Format: Patent
• Language: Chinese; English
• Published: 28.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a primer group and a method for detecting mutation of 108 polymorphic sites of deafness related genes. A primer group is designed aiming at gene segments where 108 mutation sites of 18 genes related to hereditary hearing loss are located, an amplicon capture sequencing method is adopted, the primer group is firstly utilized to perform multiple PCR technology amplification and construct a sequencing library, then a new generation of high-throughput sequencing platform Illumina miniseq is combined to perform detection, and by analyzing all sequencing signals, the mutation sites of the 18 genes related to hereditary hearing loss can be detected, so that the mutation sites of the 18 genes related to hereditary hearing loss can be detected, and the mutation sites of the 18 genes related to hereditary hearing loss can be detected. The relative quantification of different basic groups at each site of the DNA sequence and the sequence judgment of DNA fragments are realized. The method is easy