Vitamin D receptor gene SNP (Single Nucleotide Polymorphism) detection kit and application

• Main Authors: ZHANG YANCHI, WANG LIWU, SUN LIWEI, MA YINGWEI
• Format: Patent
• Language: Chinese; English
• Published: 28.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a vitamin D receptor gene SNP (Single Nucleotide Polymorphism) detection kit which comprises a primer of a vitamin D receptor gene; the vitamin D receptor gene is a vitamin D receptor FokI gene; a vitamin D receptor gene SNP detection system comprises the vitamin D receptor gene SNP detection kit, and a PCR reaction system comprises 4 [mu] L of template DNA, 4 [mu] L of 5HS Taq buffer, 0.5 [mu] L of F Primer, 0.5 [mu] L of R Primer, 0.1 [mu] L of hot start Taq DNA polymerase, 0.4 [mu] L of dNTP, and the total volume is fixed to 20 [mu] L by using sterilized water. The SNP of the VDR gene of a DLD child patient is detected and analyzed, and the result shows that DLD is related to the VDR FokI gene; the occurrence and the development of the DLD are related to the SNP (Single Nucleotide Polymorphism) of the VDR FokI gene. 本发明公开了维生素D受体基因SNP检测试剂盒，它包括，所述维生素D受体基因的引物；所述的维生素D受体基因为维生素D受体FokI基因；维生素D受体基因SNP检测体系，它包括：所述的维生素D受体基因SNP检测试剂盒，PCR反应体系为：模板DNA 4μL，5´HS Taq buffer 4μL，F Primer 0.5μL，R Primer