Construction method of cyanogen hydratase strain and application of cyanogen hydratase strain in nicotinamide production

• Main Authors: WANG BENZHU, XU BIN, ZHUO XUERUI, FANG GUOKANG, JI CHUANXIA, WANG SHU
• Format: Patent
• Language: Chinese; English
• Published: 28.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a genetically engineered bacterium. The genetically engineered bacterium provided by the invention can efficiently express soluble nitrile hydratase in a wide temperature range, and the obtained enzyme has high activity. When the genetically engineered bacterium is used for producing the nitrile hydratase, low-temperature induction is not needed in the fermentation process, energy consumption is reduced, and the obtained nitrile hydratase can efficiently catalyze nicotinonitrile to prepare nicotinamide. 本发明提供了一种基因工程菌。本发明提供的基因工程菌，能够在宽泛的温度范围内高效表达可溶性腈水合酶，获得的酶活性高。利用该基因工程菌生产腈水合酶，发酵过程中无需低温诱导，减少能耗，并且获得的腈水合酶可以高效催化烟腈制备烟酰胺。