Plant species identification method based on whole genome analysis and genome editing and application

• Main Authors: SONG KYOUNG-WON, XIN TIANYI, XU WENJIE, QI GUIHONG, HAO LIJUN
• Format: Patent
• Language: Chinese; English
• Published: 24.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses a plant species identification method based on whole genome analysis and genome editing and application, namely a GAGE method (Genome Analysis and Genome Editing), the method specifically comprises the following steps: screening a sequence with PAM (Polyacrylamide) in a whole genome of a plant to be identified; the method comprises the following steps of: comparing the Cas protein with genomes of adulterants and closely related species, selecting a sequence only existing in a plant genome to be identified as a target sequence, introducing the target sequence into a genome editing system to detect the target sequence, designing and synthesizing crRNA (CRISPR RNA) according to the selected target sequence, guiding the Cas protein to be combined with the target sequence to form a complex by the crRNA, and detecting the Cas protein by using the target sequence. Then, the trans-cleavage activity of the Cas protein is activated, the single-stranded DNA with the fluorescence signal group is c