Chromatographic microscopic imaging device and method based on resonance scanning sparse structured light illumination

• Main Authors: AN SHA, CHEN XIAOFEI, GAO PENG, ZHENG JUANJUAN, LIU XING, LEI YUNZE
• Format: Patent
• Language: Chinese; English
• Published: 24.03.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a chromatography microscopic imaging device and method based on resonance scanning sparse structured light illumination. The device comprises an illumination unit, a scanning unit, a microscopic imaging unit and an acquisition and reconstruction control unit, wherein the illumination unit generates illumination light of which the light intensity changes along with the spatial position under the control of a control signal generated by the acquisition and reconstruction control unit; the scanning unit completes generation and phase shift of sparse scanning stripe structured light through focus scanning by utilizing a control signal generated by the acquisition and reconstruction control unit; the microscopic imaging unit is used for acquiring intensity images of different axial slices of the sample under the irradiation of the sparse scanning stripe structured light with different phases by utilizing a control signal generated by the acquisition and reconstruction control unit; and the