Detection method for detecting porcine epidemic diarrhea virus, brachyspira hyodysenteriae and lawsonia intracellularis

• Main Authors: ZU CONGCONG, YU QIANNAN, WANG YUNZHOU, LI YANG, QIAO MENGLI, LI FUJUN, LI XIAOWEN
• Format: Patent
• Language: Chinese; English
• Published: 24.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a detection method for detecting porcine epidemic diarrhea virus, brachyspira hyodysenteriae and lawsonia intracellularis. According to the detection method, the simultaneous detection of the porcine epidemic diarrhea virus, the brachyspira hyodysenteriae and the lawsonia intracellularis is realized by utilizing primers and probes for detecting the porcine epidemic diarrhea virus, the brachyspira hyodysenteriae and the lawsonia intracellularis. According to the method provided by the invention, the detection cost is saved, the time is saved, the detection of the three pathogens is completed in one reaction system, and aerosol pollution is avoided to a great extent. 本发明提供了一种用于检测猪流行性腹泻病毒、猪痢疾短螺旋体和猪胞内劳森菌的检测方法。该检测方法通过利用检测猪流行性腹泻病毒、猪痢疾短螺旋体、胞内劳森菌的引物和探针实现猪流行性腹泻病毒、猪痢疾短螺旋体、胞内劳森菌的同时检测。本发明提供的方法节省了检测成本，节约了时间，且三种病原的检测均在一个反应体系中完成，极大限度的避免了气溶胶污染。