Strain and method for producing beta-elemene

• Main Authors: BAI YAJUN, DING YANRUI, CAI YUJIE, HU XIAOXIANG, ZHENG XIAOHUI
• Format: Patent
• Language: Chinese; English
• Published: 24.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a strain and a method for producing beta-elemene, and belongs to the technical field of bioengineering. According to the invention, recombinant cells or a combination of recombinant cells for expressing farnesol kinase, farnesyl phosphokinase, polyphosphate kinase 2 and gemmene A synthase are constructed, and the recombinant cells or the combination of the recombinant cells are utilized to catalyze farnesol to synthesize beta-elemene. The substrate provided by the invention is low in price and easy to obtain, and 17g/L beta-elemene can be obtained by adding 20g/L farnesol and reacting for 24h. Therefore, the method has a good industrial application prospect, and large-scale production of the beta-elemene is facilitated. 本发明公开了一种生产β-榄香烯的菌株及方法，属于生物工程技术领域。本发明构建了表达法尼醇激酶，法尼基磷酸激酶，多聚磷酸激酶2，吉玛烯A合酶的重组细胞或者重组细胞的组合，并利用重组细胞或者重组细胞的组合催化法尼醇合成β-榄香烯。本发明的底物价格低廉，容易获得，且本发明在添加20g/L法尼醇反应24h可以获得17g/L的β-榄香烯。因此，本发明具有良好的工业化应用前景，有助于实现β-榄香烯的大规模生产。