Method for dissociating banana radicle protoplast

The invention relates to a banana radicle protoplast dissociation method, and belongs to the technical field of plant cell protoplast separation. The method comprises the following steps: selecting radicle tissues germinating for 14 days as a material, cutting the radicle tissues into slices, adding...

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Bibliographic Details
Main Authors PANG MEIXIA, CHEN ZHIHAO, LIN CHUANYONG, WU SHENGPENG
Format Patent
LanguageChinese
English
Published 21.03.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a banana radicle protoplast dissociation method, and belongs to the technical field of plant cell protoplast separation. The method comprises the following steps: selecting radicle tissues germinating for 14 days as a material, cutting the radicle tissues into slices, adding enzymatic hydrolysate, and carrying out oscillation enzymolysis under a dark condition for 1-3 hours; and after enzymolysis, filtering by using a 70-micron cell filter and a 40-micron cell filter, cleaning by using a protoplast buffer solution, and carrying out low-temperature low-speed centrifugal precipitation to obtain the protoplast. According to the method, the banana radicle protoplast dissociation is realized, the operation is simple and convenient, the protoplast can be rapidly and efficiently prepared, the yield of the protoplast separated according to the optimal enzyme concentration is as high as 1.2 * 10 < 6 >/mL and the motility rate is as high as 92% after FDA dyeing and detection of a fluorescent in
Bibliography:Application Number: CN202211581158