Fusion protein containing salinity-induced self-assembly peptide and application of fusion protein in recombinant protein purification

• Main Authors: YANG XIAOFENG, LIN ZHANGLIN, LIU RUN, JUNG IN JIN
• Format: Patent
• Language: Chinese; English
• Published: 21.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a fusion protein containing a salinity-induced self-assembly peptide and an application of the fusion protein in recombinant protein purification. The polypeptide is at least one of EFK8 polypeptide and 18 mutants derived from the EFK8 polypeptide, and the amino acid sequences of the EFK8 polypeptide are respectively shown as SEQ ID NO: 1-19. According to the self-assembled polypeptide disclosed by the invention, the fusion protein can be specifically aggregated or depolymerized through simple salinity change, the separation and purification of the recombinant protein can be realized through simple operation of centrifugation or filtration, and the self-assembled polypeptide can be used as an efficient, simple and low-cost purification method for the recombinant protein and has a good commercial application prospect. 本发明涉及一种包含盐度诱导自组装肽的融合蛋白及其在重组蛋白纯化中的应用。所述的多肽为EFK8多肽及由其衍生的18种突变体中的至少一种，其氨基酸序列分别如：SEQ ID NO：1-19。本发明的自组装多肽，可通过简单的盐度改变而使所融合蛋白能特异性地聚集或解聚，而能通过离心或过滤的简便操作实现重组蛋白的分离纯化，可作为重组蛋白高效、简便、低