Ethanol-free elution method and application of ethanol-free elution method in library construction sequencing

• Main Authors: XI YANG, ZHAO XIA, FU SHUJIN, HU DOU
• Format: Patent
• Language: Chinese; English
• Published: 17.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides an ethanol-free elution method and application, and relates to the technical field of gene sequencing, the method comprises the following steps: providing a conjugate of a magnetic bead-to-be-eluted sample; and contacting an eluent with the conjugate to elute the sample, where no ethanol is used in the elution. According to the ethanol-free elution method and the application provided by the invention, an ethanol washing step is omitted in each purification step based on the magnetic beads, so that the operation time is effectively shortened, the operation steps are simplified, and the database building quality and the data stability of subsequent sequencing can be ensured. 本申请提出了一种无乙醇洗脱的方法及应用，涉及基因测序技术领域，该方法包括：提供磁珠-待洗脱样品的结合物；和将洗脱液与所述结合物接触，以洗脱所述样品，其中，所述洗脱中不使用乙醇。本申请提出的无乙醇洗脱的方法及应用在各个基于磁珠进行的纯化步骤中省去了乙醇洗涤的步骤，有效减少了操作时间并简化了操作步骤，并且能够保证建库质量和后续测序的数据稳定性。