Genetic engineering strain of zymomonas mobilis and application thereof

• Main Authors: GENG BINAN, YANG SHIHUI, LI MIAN, HE QIAONING, DU JUN
• Format: Patent
• Language: Chinese; English
• Published: 17.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a genetic engineering strain of zymomonas mobilis and application of the genetic engineering strain. The genetic engineering strain is a strain obtained by knocking out four endogenous plasmids, namely, pZM32, pZM36, pZM33 and pZM39, in a ZM4-Cas12a strain. The genetic engineering strain ZMNP obtains a simple genome, reduces energy consumption, has a series of excellent properties, such as high conversion efficiency, more tolerance to inhibitors, better utilization ability to secondary mother liquor and the like, and is very suitable for being used as a chassis cell of industrial microorganisms. 本申请公开了一种运动发酵单胞菌的基因工程菌株及其应用。该基因工程菌株是将ZM4-Cas12a菌株中pZM32、pZM36、pZM33、pZM39四个内源性质粒敲除得到的菌株。该基因工程菌株ZMNP获得了一种精简的基因组，减少能量消耗，并且具有一系列的优良性状，如转化效率高、更加耐受抑制物、对二次母液的利用能力更好等优异性能，十分适合作为工业微生物的底盘细胞。