Saccharomyces cerevisiae engineering bacterium for improving gene expression level as well as construction method and application of saccharomyces cerevisiae engineering bacterium

• Main Authors: MENG XIANGFENG, XU MINGYUAN, ZHANG WEIXIN, LIU WEIFENG
• Format: Patent
• Language: Chinese; English
• Published: 17.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a saccharomyces cerevisiae engineering bacterium for improving a gene expression level as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. Saccharomyces cerevisiae W303-1a is used as an original strain, an extracellular protease coding gene bar1 and a galactose metabolic pathway inhibiting factor coding gene gal80 are knocked out from the saccharomyces cerevisiae engineering strain, and the saccharomyces cerevisiae engineering strain contains an alpha factor coding gene kmf alpha 1 expressed by a promoter Pvrg4 and a transcription activator coding gene gal4 expressed by a promoter Pfuse 1. The saccharomyces cerevisiae engineering bacterium for improving the gene expression level, provided by the invention, contains a gene expression regulation and control system, can be used for regulating and controlling heterologous product pathway-related enzymes including glycosyltransferase, and can achieve the effect of improving the synthesis