Methods and systems for detecting pathogens

• Main Authors: WAGNER KIM, KRUEGER BENJAMIN, BARNES ADRIAN
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Methods, compositions, and systems for detecting the presence or absence of a pathogen in a sample are disclosed. The method can include the steps of obtaining a sample from the subject and heat treating the sample to inactivate any pathogen present in the sample. The method may also include the step of processing the sample to concentrate any pathogen present in the sample. Likewise, the method may include isolating the pathogen-specific nucleic acid from the thermally inactivated sample and detecting the presence or absence of the isolated pathogen-specific nucleic acid. In certain embodiments, methods and/or systems may be used to detect SARS-CoV-2. The method can use real-time RT-PCR to provide a result in a period of about 3 hours or less. 本发明公开了检测样品中病原体是否存在的方法、组合物和系统。该方法可包括从受试者获得样品和用热处理样品以灭活样品中存在的任何病原体的步骤。该方法还可包括处理样品以浓缩样品中存在的任何病原体的步骤。同样，该方法可包括从热灭活的样品中分离病原体特异性核酸和检测分离的病原体特异性核酸是否存在。在某些实施方案中，方法和/或系统可用于检测SARS-CoV-2。该方法可利用实时RT-PCR在约3小时或更短的时间内提供结果。