Methods, systems, and compositions for nucleic acid sequencing

• Main Authors: FRANCAS, ALAIN, WU XIAOLIN, ROMANOV NIKOLAI NIKOLAEVICH, LIU XIAOHAI, CRESINA ELENA, MCCAULEY PATRICK
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present disclosure relates to methods, systems, kits, and compositions for nucleic acid sequencing applications. In particular, the method utilizes two imaging events with different excitation wavelengths and a single emission channel to collect fluorescence signal patterns of different types of nucleotide conjugates to determine the identity of incorporated nucleotide conjugates. The methods described herein do not require a chemical treatment of the nucleotide conjugate incorporated into the mixture between the two imaging events. 本公开涉及用于核酸测序应用的方法、系统、试剂盒和组合物。具体地，该方法利用具有不同激发波长的两个成像事件和单个发射通道来收集不同类型的核苷酸缀合物的荧光信号模式以确定掺入的核苷酸缀合物的同一性。本文所述的方法不需要在该两个成像事件之间对掺入混合物中的核苷酸缀合物的化学处理。