DNA polymerase variants with improved discrimination of genetic variations

• Main Authors: KIM SEUL-KI, LIM SI-KYU, KIM JAE-JONG, CHA SUN-HO, RYU JUNG-HYUN, LI FUMEI
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a DNA polymerase variant belonging to family A and a use thereof, and the present invention relates to a DNA polymerase variant which smoothly polymerizes when the base at the 3'end of a primer is complementary to a template, and which suppresses the polymerization when the base at the 3 'end of the primer is not complementary to the template, thereby easily discriminating these two situations. The invention also relates to a PCR method using the variant and a PCR kit comprising the variant. The present invention is useful for single nucleotide polymorphism analysis (SNP genotyping), somatic mutation detection, and the like. 本发明涉及一种属于A家族的DNA聚合酶变体及其用途，并且本发明涉及一种DNA聚合酶变体，当引物3'末端的碱基与模板互补时，顺利聚合，当引物3'末端的碱基与模板不互补时，聚合被抑制，从而容易辨别这两种情况，而且本发明涉及一种利用所述变体的PCR方法及包含所述变体的PCR试剂盒。本发明对单核苷酸多态性分析(SNP基因分型)及体细胞突变检测等有用。