Component blood detection method, component blood detection sheet and component blood detection device

• Main Authors: LU FEI, NI DANDAN, YAN XU, LI LINGJUN
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a component blood detection method which comprises the following steps: mixing a component blood sample with a hemolytic agent and a coloring agent for reaction to obtain a to-be-detected sample; the method comprises the following steps: filling a to-be-detected sample into a transparent chamber with the thickness of 100-150 microns and the effective detection area of more than 300mm < 2 > for fluorescence imaging to obtain a fluorescence microscopic image of white blood cells; and detecting the residual white blood cells in the component blood sample according to the fluorescent microscopic image of the white blood cells. The residual white blood cells in the component blood can be detected, and the counting is convenient and accurate. The invention also provides a detection sheet and a detection device for realizing the component blood detection method. 本发明提供一种成分血检测方法，包括：将成分血样本与溶血剂和染色剂混合反应后，获得待检测样本；将待检测样本填充在厚度为100-150μm、有效检测区域面积大于300mm2的透明腔室内进行荧光成像，获得白细胞的荧光显微图像；根据白细胞的荧光显微图像，对成分血样本中的残留