Gene chip and kit for detecting parasites of aquatic animals and application

• Main Authors: LI JIAQIAO, LIU HONG, LI HONGQUAN, SI ZEEN, ZHENG SHUYIN, ZHENG SHU, LIU XIAO, XIE YANHUI, ZHENG XIAOCONG, ZHANG NA
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a gene chip for detecting parasites of aquatic animals. The gene chip comprises a plurality of detection areas and a plurality of spacing areas for separating two adjacent detection areas, each detection area comprises a sample adding hole, a plurality of film bases and a plurality of microgrooves for communicating each film base to the sample adding hole, and a primer group specifically aiming at a characteristic target gene of an aquatic animal parasite is fixed on each film base. According to the invention, multiple aquatic product parasites can be detected synchronously, the accuracy and precision can be guaranteed, the detection efficiency is improved, and the detection time and cost are saved. 本发明提供一种用于检测水生动物寄生虫的基因芯片，其包括若干检测区和将相邻的两个检测区隔开的若干间隔区；每一检测区包括加样孔、若干片基，以及将每一个片基连通至加样孔的若干微槽，每一片基上分别固定有特异性针对一种水生动物寄生虫的特征性靶基因的引物组。本发明能够对多种水产品寄生虫同步进行检测，且能够保障准确度和精确性，提升了检测效率，节省了检测时间和成本。