Fish scale antifreeze peptide as well as bioinformatics-based screening method and application thereof

• Main Authors: WANG SHAOYUN, CAI QIANQIAN, CHEN XU, YANG FUJIA, HUANG JIANLIAN, JIANG WENTING
• Format: Patent
• Language: Chinese; English
• Published: 14.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of polypeptide, in particular to fish scale antifreeze peptide as well as a bioinformatics-based screening method and application thereof. The fish scale antifreeze peptide has an amino acid sequence as follows: IAQPQEKAPDPY; the sites for forming hydrogen bonds with ice crystals comprise GLU6, ASP10 and TVR12. The screening method comprises the following steps: carrying out mass spectrometric analysis on the primary extract of the antifreeze peptide from the fish scales of the opuntia paliurus to obtain a peptide sequence; screening the peptide sequence and an ice crystal edge surface through molecular docking software to obtain an optimal conformation peptide sequence; and screening the antifreeze peptide sequence from the optimal conformation peptide sequence by taking the number of formed hydrogen bonds as an index under three-dimensional analysis. The antifreeze peptide provided by the invention has high antifreeze activity, and can be applied as an antifreeze