Liquid drop micro-fluidic chip prepared based on 3D printing and SERS (Surface Enhanced Raman Scattering) detection method

• Main Authors: CHEN SIHONG, WU LEI, WANG ZHUYUAN, LU SIYUAN, YANG JUN, YANG KUO
• Format: Patent
• Language: Chinese; English
• Published: 07.03.2023
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; TESTING; TRANSPORTING

The invention discloses a liquid drop micro-fluidic chip prepared based on 3D printing and an SERS detection method, the micro-fluidic chip is made of a photosensitive resin material by using a 3D printer, and the liquid drop micro-fluidic chip comprises a cross-shaped liquid drop generation area (1), a spiral mixed reaction area (2) and a liquid drop collection area (3). Micro-droplets can be generated in a chip channel by adjusting the flow velocity of a water-oil two-phase solution, metal nanoparticles and a to-be-detected object are fully mixed in the micro-droplets, and qualitative and quantitative analysis of the to-be-detected object is realized by collecting an SERS spectrum in a droplet collection area. The liquid drops move in the three-dimensional channel, so that the mixing efficiency of the metal nanoparticles and an object to be detected can be improved, and rapid and high-sensitivity SERS detection is realized. 本发明公开了一种基于3D打印制备的液滴微流控芯片及SERS检测方法，所述微流控芯片采用光敏树脂材料，利用3D打印机制做而成，所述液滴微流控芯片包括十字型液滴生成区(1)