Insect nicotinamide enzyme inhibitor, identification method and application of insect nicotinamide enzyme inhibitor to insecticide

• Main Authors: QIAO XIAOMU, HUANG JIA
• Format: Patent
• Language: Chinese; English
• Published: 07.03.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PHYSICS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

The invention discloses an insect nicotinamide enzyme inhibitor, an identification method and an application of the insect nicotinamide enzyme inhibitor to insecticides, and the method comprises the following steps: providing an insect nicotinamide enzyme protein which is expressed and purified in vitro, determining the activity of the insect nicotinamide enzyme protein, and screening the active insect nicotinamide enzyme protein by detecting whether nicotinic acid or ammonia can be obtained through reaction. Adding a candidate compound into the screened active insect nicotinamide enzyme protein, determining the activity of the insect nicotinamide enzyme protein in the presence of the compound, and the like. The invention provides a molecular target, namely insect nicotinamide enzyme, which can be used for developing new pesticides. As a brand new target of an insecticide and application of the compound as the insecticide, the compound is an innovative insecticide target found for the first time in the world