Method for determining interaction between nucleic acid aptamer and small molecule target based on surface enhanced Raman spectroscopy

• Main Authors: WANG SHUANGSHUANG, LI HUIHUI, DAI YUANLI, WANG SHUANGHAO
• Format: Patent
• Language: Chinese; English
• Published: 03.03.2023
• Subjects: CASTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MAKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MEASURING; NANOTECHNOLOGY; PERFORMING OPERATIONS; PHYSICS; POWDER METALLURGY; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; TESTING; TRANSPORTING; WORKING METALLIC POWDER

The invention discloses a method for determining interaction between a nucleic acid aptamer and a small molecule target based on surface enhanced Raman spectroscopy, which comprises the following steps: preparing high-activity nano-silver colloid particles by using a method for reducing silver nitrate by hydroxylamine hydrochloride at room temperature, and mixing the nano-silver colloid particles as an enhancing reagent with a sample solution; by analyzing the surface enhanced Raman spectrum signal change before and after the combination of the nucleic acid aptamer and the small molecule target, the influence of target combination on the space structure of the nucleic acid aptamer is distinguished, the method has the characteristics of high analysis speed and high sensitivity, and a new strategy and a new idea are provided for the screening, design and structure optimization of the nucleic acid aptamer. 本发明公开了一种基于表面增强拉曼光谱测定核酸适配体与小分子靶标间相互作用的方法，通过在室温下用盐酸羟胺还原硝酸银的方法制备高活性的纳米银胶颗粒，并将其作为增强试剂与样品溶液混合，通过分析核酸适配体和小分子靶标结合前