Method for constructing recombinant insecticidal escherichia coli to express mosquito poison protein

• Main Authors: ZHANG QI, WANG JINGYI, SUN YILIN, LU DI, ZHANG PORUI, LI XUEHAI
• Format: Patent
• Language: Chinese; English
• Published: 03.03.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention discloses a method for constructing recombinant insecticidal escherichia coli to express mosquito poison protein, which comprises the following steps: by taking escherichia coli as host bacteria, inserting a coding gene of bacillus thuringiensis Cry11Ba between BamHI and NdeI restriction enzyme cutting sites of a pET-30a vector to obtain a recombinant vector, and introducing the recombinant vector into the host bacteria to obtain the mosquito poison protein. The recombinant insecticidal Escherichia coli can be massively fermented through a fermentation tank to obtain commercial wettable powder in the forms of missible oil, dry powder and the like, has the effects of high specificity and high insecticidal efficacy on pests, and is friendly to the environment, people and livestock; the strain has high toxicity to mosquitoes in disease transmission media, and is an important biological control strain for field and urban mosquito control. The strain is high in growth capability, high in toxin prepar