HPV6 type capsid protein L1 monoclonal antibody as well as preparation method and application thereof

• Main Authors: ZHANG JIANCHENG, RUAN BAOYANG, ZHU JUNLI, CAO YUFENG, WANG XINFENG, LIU LIN, YAN JIACHENG, SHI LI
• Format: Patent
• Language: Chinese; English
• Published: 03.03.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to the technical field of monoclonal antibodies, in particular to an HPV6 type capsid protein L1 monoclonal antibody as well as a preparation method and application thereof. The invention provides a binding molecule specifically binding to HPV6 type capsid protein L1, a mouse monoclonal antibody aiming at type 6 HPV is obtained by screening through a hybridoma technology, and the antibody has high specificity and neutralizing activity on HPV6L1VLPs and pseudoviruses. A double-antibody sandwich indirect ELISA method is established based on the monoclonal antibody, a rabbit polyclonal antibody and an anti-mouse IgG enzyme-labeled antibody and is used for detecting the HPV6L1 antigen. The method has good specificity, sensitivity, linearity and accuracy, evaluates the content and activity of the effective antigen component of the vaccine, and has important values for production of the HPV vaccine and prevention and control of diseases such as condyloma acuminate, verruca plana and recurrent