Reference ladder and adapter

• Main Author: MERCER TODD
• Format: Patent
• Language: Chinese; English
• Published: 03.02.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DERIVATIVES THEREOF; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; SUGARS; VINEGAR; WINE

The present disclosure generally relates to polynucleotides that can be used in calibration methods that determine the identity and/or quantity of polynucleotides in a sample. In particular, the present disclosure relates to polynucleotides comprising a calibration sequence for use as a reference ladder or a reference adaptor. The polynucleotides can be used to calibrate a wide variety of sequencing methods, including high throughput sequencing methods (e.g., those referred to as next generation sequencing or NGS methods). The disclosure also generally relates to the use of these polynucleotides in a wide variety of applications, including, for example, in calibration of a wide variety of sequencing methods. 本公开总体上涉及可用于确定样品中多核苷酸的身份和/或数量的校准方法的多核苷酸。特别地，本公开涉及包含用作参考梯状物或参考衔接子的校准序列的多核苷酸。所述多核苷酸可用于校准各种各样的测序方法，包括高通量测序方法(例如，称为下一代测序或NGS方法的那些方法)。本公开还总体上涉及这些多核苷酸在各种各样的应用中，包括例如在各种各样的测序方法的校准中的用途。