Method for simultaneously determining contents of various fructus psoraleae substances in lung-tonifying and blood-activating capsule by adopting quantitative analysis of multi-components by single marker

• Main Authors: HU YANGYE, WANG HENGBIN, GENG WANLI, WANG ZHONGKUAN, LI QUAN, ZOU ZHIHUI
• Format: Patent
• Language: Chinese; English
• Published: 03.02.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the field of traditional Chinese medicines, in particular to a method for simultaneously determining the contents of various fructus psoraleae substances in lung-tonifying and blood-activating capsules by adopting a QAMS (quantitative analysis of multi-components by single marker) method. The lung-tonifying and blood-activating capsule comprises astragalus membranaceus, radix paeoniae rubra and fructus psoraleae, and the method comprises the following steps: taking psoralen as an internal reference substance, and respectively determining relative correction factors of psoralen glycoside, isopsoralen glycoside, isopsoralen and bakuchiol relative to psoralen; a test solution is obtained, wherein the test solution contains the content of the lung tonifying and blood circulation promoting capsule; and carrying out high performance liquid chromatography detection on the test solution based on a relative correction factor of psoralen, isopsoralen, isopsoralen or bakuchiol relative to psoral